Phytochemical analysis and antimicrobial activity of water melon seed extract in Streptococcus SPP Department: Microbiology By: Ziggerzillion Project ID: 7102 Rating: (5.0) votes: 1Rate this project12345 Price:₦2000 Get the Complete MaterialAbstractWatermelon seed was evaluated for its phytochemical and antimicrobial potential. Crude extract of the seeds was obtained using hot water, cold water, ethanol and methanol. Test organisms were screened to confirm their viability and identities using standard microbiological methods. Extracts were tested for antimicrobial activity using the standard disc diffusion assay method against Staphylococcus Aureus, Pseudomonas Aeruginosa, Escherichia coli, Klebsiella Pneumoniae and Bacillus cereus. All the seed extracts showed evidence of antibacterial properties. Hot water extract showed the highest antimicrobial activity against Pseudomonas aeruginosa with 14mm diameter zone of inhibition whereas ethanol and methanol extracts showed the lowest against Escherichia coli and Klebsiella pneumoniae with 8mm diameter zone of inhibition. Watermelon seed showed low antimicrobial activity when compared to the result of the commercial antibiotics. The analysis for phytochemical constituents was performed using generally accepted laboratory techniques for quantitative determinations. The constituents analyzed for were tannins, saponins, flavonoids, cyanogenic glycosides, oxalates and alkaloids. Alkaloid had the highest concentration of about 1.23% whereas cyanogenic glycoside had the lowest of about 0.00237%. There was a correlation between the phytochemical levels and the antimicrobial activities. The low level of phytochemicals explains the low antimicrobial activities of extracts of watermelon seeds. ...Preview Download Preview +Other Microbiology project topics and materials you might be interested in»Isolation and identification of microbes associated with spoilage bread»Bacterial analysis of urine polluted environment in federal polytechnic Nekede, Owerri»Microbiological quality of palm kernel fruits»Microbiological evaluation of home made grape juice»Therapeutic potential of Moringa Oleifera leaves»Antibacterial activity of honey on staphylococcus aureusescherichia coli and streptococcus pyogen isolated from wound»Bacteriological monitoring of abattoirs in Ehime Mbano in Imo state»Phytochemical screening and antimicrobial assessment of Spondias Mombin (Ijikara)»Proximate, selected vitamins, and minerals analysis of Moringa Oleifera»Biodeterioration of natural and artifical stone»Isolation and identification of bacterial aerosols bioaerosols dump sites in Yenagoa»The effect of crude avocado and pawpaw leaves extract on kidney function of guinea pigs»Microbial examination of raw meat obtained at main abattoir, Offa»Isolation and characterization of fungi associated with the spoilage of corn grown in farms in Rukpokwu community»Incidence of staphylococcus and salmonella species in Kunuaya retailed in Yenagoa