Detection, quantification and antibiotic resistance of entero-pathogenic bacteria of hospital waste water in Akad Private Hospital, Okitipupa, Ondo state Department: Microbiology By: SIRJOSEPH Project ID: 8809 Rating: (5.0) votes: 1Rate this project12345 Price:₦4000 Get the Complete MaterialAbstractEnteropathogens are a specific group of pathogenic microorganisms that cause infections in the intestinal tract, typically leading to gastrointestinal illnesses such as diarrhea, dysentery, typhoid, and cholera. The aim of this study is to detect and quantify enteropathogenic bacteria in hospital wastewater. Sample were collected from the Akad Private Hospital Okitipupa in Ondo State, Nigeria. Samples were analyzed using conventional and standard microbiological techniques. Results revealed that 7.0x105CFU/mL, 5.6x106 CFU/mL, 4.6x107 CFU/mL, and 1100MPN/100mL of Escherichia was present in the sample, which indicates fecal contamination. Biochemical identification revealed enteropathogenic organisms such as Escherichia coli, Klebsiella ornithinolytica and Salmonella typhi. Antibiotic sensitivity testing was performed using the disc diffusion method in accordance with Clinical and Laboratory Standards Institute (CLSI) guidelines. The results demonstrated a high prevalence of multidrug resistance among the bacterial isolates. Considerable resistance was recorded against augmentin, levofloxacin, gentamicin and vancomycin. A number of strains exhibited intermediate resistance to amikacin, nitrofurantoin and ciprofloxacin, indicating reduced effectiveness of these agents. Nevertheless, some isolates remained susceptible to aztreonam, cefepime, meropenem, cotrimoxazole and ceftriaxone. Hospital wastewater is not just an environmental pollutant but also a potential public health hazard, especially in resource-limited settings where wastewater treatment infrastructure is inadequate. Hospitals should adopt effective wastewater treatment to reduce the spread of enteropathogenic and antibiotic-resistant bacteria. Routine monitoring of effluents is essential for early detection and control. Antibiotic use must be regulated through stewardship programs to minimize resistance. Environmental regulations should be enforced, and awareness campaigns should educate both staff and the public...Preview Download Preview +Other Microbiology project topics and materials you might be interested in»Isolation and identification of microbes associated with spoilage bread»Bacterial analysis of urine polluted environment in federal polytechnic Nekede, Owerri»Microbiological quality of palm kernel fruits»Microbiological evaluation of home made grape juice»Therapeutic potential of Moringa Oleifera leaves»Antibacterial activity of honey on staphylococcus aureusescherichia coli and streptococcus pyogen isolated from wound»Phytochemical screening and antimicrobial assessment of Spondias Mombin (Ijikara)»Bacteriological monitoring of abattoirs in Ehime Mbano in Imo state»Proximate, selected vitamins, and minerals analysis of Moringa Oleifera»Biodeterioration of natural and artifical stone»The effect of crude avocado and pawpaw leaves extract on kidney function of guinea pigs»Isolation and characterization of fungi associated with the spoilage of corn grown in farms in Rukpokwu community»Microbial examination of raw meat obtained at main abattoir, Offa»Isolation and identification of bacterial aerosols bioaerosols dump sites in Yenagoa»Incidence of staphylococcus and salmonella species in Kunuaya retailed in Yenagoa