Molecular or identification studies of biosufactant producing micro-organisms from environment samples Department: Science Technology By: GodFirst Project ID: 5736 Rating: (5.0) votes: 1Rate this project12345 Price:₦2000 Get the Complete MaterialAbstractIsolation and screening of microorganisms from contaminated soil samples with biosurfactant producing ability were investigated. The biosurfactant producing ability of the microorganisms isolated from the soil samples was investigated by hemolytic assay, drop collapse test, emulsification index and methylene blue agar plate method. Biosurfactants are biodegradable, non-toxic and eco-friendly compounds released by microorganisms. Most microorganisms were isolated from contaminated sites by petroleum hydrocarbon and industrial wastes. In this study, a biosurfactant–producing strain. Nigeria by enrichment culture, hemolytic activity assay, hydrolyzing oil activity assay, improved degreasing effect assay, and emulsification activity measurement. The strain was identified as pseudomonas Sp. BSI based on its 165 RNA gene sequence. The identified isolated is capable of producing glycolipids or other anionic surfactants as determined in our analysis of a phenotypic assay using CTAB. The biosurfactant was isolated from the culture supernatant and identified as dirhamnolipids (Rha-Rha-c10-c10) by high-performance liquid chromatography-mas spectrometry. The culture filtrate and cell-free supernatant that the isolate potential use in enhanced oil recovery. The dominant species were Bacillus and Pseudomonas, with an occurrence rate of 22.73% each. The isolates were subjected to conventional biosurfactant screening tests: qualitatively (drop collape and microplate assay) and quantitatively (oil spreading and emulsification activity). Therefore Bacillus and Pseudomonas species were positive for all the tests and they had a cleaning zone of 4mm each and an emulsification capacity of 51.61% and 53.13% respectively. ...Preview Download Preview +Other Science Technology project topics and materials you might be interested in»Bacteriological qualities of food thickener deuterium microcarpum (ofor) and brachystegia eurcycoma (achi) sold in Owerri main market»Obstacle detection robot with ultrasonic sensor»Design and construction of cell phone charger»Physiochemical assessment of soil samples from Nwambe Reserve Forest, Ndubia Igagu Izzi L.G.A, Ebonyi State.»Antitrypanosomal activity of ethanol extract of Tridax Procumbent leaf and whole flower on T. Brucei infected rats (Invitro and Invivo)»Pharmacological activities of plant secondary metabolites»A study on the effects of beer on the efficacy of antimalarial drug administered 1hour before beer consumption in albino rats»Qualitative production of biodiesel using neem seed oil»Physico-chemical assessment of soil sample from Igbagu reserve forest in Ebonyi State»Fungi associated with the deterioration of in-can and painted walls of Akanu Ibiam Federal Polytechnic, Uwana»Theory and application of direct hydrocarbon indicators»Microbial load of some dehydrated foods with special reference to dried tomatoes and mushrooms»The assessment of surface water quality in Otamiri river in Nekede, Owerri west, Imo state»Antimicrobial properties of edible mushroom»Production of potassium salt K2So4 from agricultural waste unripe plantain peels