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Isolation and identification of bacteria from food vendors and some vegetable available at Ogbete market, Enugu state

 Department: Microbiology  
 By: usericon bapatigi7954  

 Project ID: 6940
   Rating:  (5.0) votes: 1
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   Price:₦2000
Abstract
Safety of food is a basic requirement of food quality. A total of 25 street food samples (Jollof rice, Egwusi soup, Ugu, Waterleaf and green), were randomly purchased from five different vendors in Ogbete main market Enugu. The samples were transported in ice to the laboratory. The samples were bacteriologically analyzed using the pour plate technique and sub-culture.  Pour plate techniques was done by carrying out serial dilution of the sample after which the first tube and the last tube were picked and 1ml of each sample was pipette into a Nutrient agar, the plate was then incubated for 24 hours at 37oC after which the plate was examined for growth. Subculture was done using bacteriological agar. All the screened food samples had varying levels of bacterial growth ranging from 1.0 X 105   to 3.0 X 106  cfu/ml. ninety percent of the sampled foods had bacterial counts above the acceptable limits (104 cfu/ml) and 10% of the samples had bacterial counts less than (104 cfu/ml). Six bacterial species were isolated from the foods sampled....
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