Antimicrobial efficacy of guava (Pisdium guajava) and orange (Citrus sinensis) stems extract against Streptococcus Mutans and Candida Albicans Department: Microbiology By: Ziggerzillion Project ID: 7103 Rating: (5.0) votes: 1Rate this project12345 Price:₦2000 Get the Complete MaterialAbstractThis research was conducted in the microbiology laboratory of Godfrey Okoye University to determine the antimicrobial efficacy of aqueous, methanol, ethanol of Pisdium guajava and Citrus x sinensis on the pathogenic Streptococcus mutans and Candida albicans. The Dimethyl sulfoxide was used for dissolving the plant extracts. Pisdium guajava showed antimicrobial activity against C. albicans with the exception of methanol showing no zone of inhibition to any of the isolates. The aqueous and methanol plant extract of Citrus x sinensis showed antimicrobial activity against S. mutans and C. albicans. Citrus x sinensis showed highest inhibition with MIC concentrations of 0.256g/ml, 0.064g/ml, 0.032g/ml showed slight growth while concentrations 0.016g/ml and 0.008g/ml showed heavy growth, there were scanty growths in the MBC and MFC plates. Phytochemical screening, proximate, chromatographic analyses and the antimicrobial activities of tender stem of Psidium guajava and Citrus x sinesis, were carried out. The phytochemical screening revealed the presence of all metabolites and compounds tested for such as flavonoids, tannins, reducing sugar, terpenes, saponins, anthraquinones and alkaloids. The antimicrobial screening of methanol extract showed activity against the tested organisms. The antimicrobial screening of ethanol and aqueous also showed activity against the tested organisms. The result indicated that the plants had a potential antimicrobial activity and was concentration dependent. The chromatographic analysis of the extracts showed the presence of a variety of compounds. This, therefore, supports the traditional medical use of Psidium guajava and Citrus x sinensis ...Preview Download Preview +Other Microbiology project topics and materials you might be interested in»Isolation and identification of microbes associated with spoilage bread»Bacterial analysis of urine polluted environment in federal polytechnic Nekede, Owerri»Microbiological quality of palm kernel fruits»Microbiological evaluation of home made grape juice»Therapeutic potential of Moringa Oleifera leaves»Antibacterial activity of honey on staphylococcus aureusescherichia coli and streptococcus pyogen isolated from wound»Bacteriological monitoring of abattoirs in Ehime Mbano in Imo state»Phytochemical screening and antimicrobial assessment of Spondias Mombin (Ijikara)»Proximate, selected vitamins, and minerals analysis of Moringa Oleifera»Biodeterioration of natural and artifical stone»Isolation and identification of bacterial aerosols bioaerosols dump sites in Yenagoa»The effect of crude avocado and pawpaw leaves extract on kidney function of guinea pigs»Microbial examination of raw meat obtained at main abattoir, Offa»Isolation and characterization of fungi associated with the spoilage of corn grown in farms in Rukpokwu community»Incidence of staphylococcus and salmonella species in Kunuaya retailed in Yenagoa