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Extraction and characterization of various prebiotics from some plant and food based sources

 Department: Microbiology  
 By: usericon Dempelove  

 Project ID: 8840
   Rating:  (5.0) votes: 1
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   Price:₦4000
Abstract
This study focused on the extraction, purification, and functional characterization of prebiotic compounds from locally sourced garlic (Allium sativum) and onion (Allium cepa). The prebiotics were extracted using Soxhlet extraction with n-hexane, followed by ethanol precipitation for purification. Antimicrobial assays were performed using the agar well diffusion method against common pathogens (Salmonella spp. and Escherichia coli) to evaluate inhibitory activity. Additionally, the potential of the extracts to support probiotic growth was assessed using Lactobacillus casei and Lactobacillus acidophilus. Results indicated that while garlic and onion oils demonstrated modest antimicrobial effects compared to the antibiotic control (ampicillin), they exhibited promising prebiotic potential through their synergistic activity with probiotic strains. Statistical analysis confirmed significant differences in antibacterial activity between the test samples and control (p  0.05). These findings suggest that garlic and onion extracts could serve as low-cost, locally sourced ingredients in the development of functional foods and nutraceuticals aimed at improving gut health and combating microbial infections. This study contributes to the growing field of natural prebiotics and supports the use of indigenous plant resources for health-promoting interventions....
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