Antibiotics susceptibility pattern of bacteria isolated from toilet seats in the female hostel of Paul University, Awka Department: Microbiology By: PRINDYTECHNIE Project ID: 8904 Rating: (5.0) votes: 1Rate this project12345 Price:₦5000 Get the Complete MaterialAbstractToilets have long been known potential vehicle for transmitting human infectious diseases. This study aimed to evaluate the antibiotic susceptibility pattern of bacteria isolated from toilet seats in the female hostel of Paul University, Awka. The samples were collected from the toilet seats using a sterile swab stick. Standard microbiological procedures were carried out to obtain pure colonies of the isolates, namely E. coli, Enterobacter sp, Bacillus sp. Staphylococcus sp, Klebsiella sp, Shigella sp and Proteus sp. Antibiotic susceptibility test was performed using Kirby-Bauer’s disc diffusion method as described in Clinical and Laboratory Standards Institute for antimicrobial disk susceptibility tests. Pure colonies of the isolates were standardized to a 0.5 MacFarland equivalent. The standardized isolates were inoculated on sterile Mueller-Hinton agar plates using swab sticks. Antibiotic discs (Ampicillin-30µg, Septrin-30µg, Augmentin-30µg, Streptomycin-30µg, Ciproflox-5µg, Gentamycin-10µg, Ciprofloxacin-10µg) were aseptically placed on the surface of the inoculated plates and incubated aerobically at 370C for 18- 24 hours. The inhibitory zone diameter was measured and interpreted as susceptible (S), intermediate (I), or resistant (R), according to the zone diameter breakpoints of the performance standards of antibiotic susceptibility testing of CLSI. E. coli and Enterobacter sp were susceptible to Ciprofloxacin, Bacillus sp and Proteus sp were susceptible to Septrin, Ciprofloxacin and Gentamycin and resistant to other antibiotics. Staphylococcus sp was susceptible to most of the antibiotics, while Klebsiella sp and Shigella sp were resistant to most antibiotics. The presence of the bacteria on the toilet seats of the female hostel showed the insanitary condition of the toilets. Regular cleaning of toilets with disinfectants and proper usage of toilets should be instructed. Modern technologies and toiletries should be provided in the public lavatory to reduce the burden of bacteria from depositing on the toilet seats....Preview Download Preview +Other Microbiology project topics and materials you might be interested in»Isolation and identification of microbes associated with spoilage bread»Bacterial analysis of urine polluted environment in federal polytechnic Nekede, Owerri»Microbiological quality of palm kernel fruits»Microbiological evaluation of home made grape juice»Therapeutic potential of Moringa Oleifera leaves»Antibacterial activity of honey on staphylococcus aureusescherichia coli and streptococcus pyogen isolated from wound»Phytochemical screening and antimicrobial assessment of Spondias Mombin (Ijikara)»Bacteriological monitoring of abattoirs in Ehime Mbano in Imo state»Proximate, selected vitamins, and minerals analysis of Moringa Oleifera»Biodeterioration of natural and artifical stone»The effect of crude avocado and pawpaw leaves extract on kidney function of guinea pigs»Isolation and characterization of fungi associated with the spoilage of corn grown in farms in Rukpokwu community»Microbial examination of raw meat obtained at main abattoir, Offa»Isolation and identification of bacterial aerosols bioaerosols dump sites in Yenagoa»Incidence of staphylococcus and salmonella species in Kunuaya retailed in Yenagoa