Effect of methamphetamine exposure on the prefrontal cortex of adult male wistar rats Department: Microbiology By: samadchuks Project ID: 9199 Rating: (5.0) votes: 1Rate this project12345 Price:₦5000 Get the Complete MaterialAbstractMethamphetamine is a powerful, highly addictive stimulant that affects the central nervous system. This research was done to discover the toxic or beneficial effects on the prefrontal cortex. Sixteen (16) adult male Wistar rats were divided into four groups of four animals each. Group A(control) was given guinea feed pellets and water, group B was administered 2mg/kg body weight of methamphetamine, group C was administered 4mg/kg body weight of methamphetamine, and group D was administered 10mg/kg body weight of methamphetamine. The administration was given daily orally for twenty-one (21) days. Twenty-four (24) hours after the last administration, the animals were weighed, then anesthetized under chloroform vapor and the animal skull dissected by occipitofrontal incision. The brain tissues were harvested from the animals and weighed. The brain tissues of some of the animals were prepared for biochemical analysis, while the remaining tissues were fixed in 10% Neutral Buffered saline for forty-eight (48) hours and grossed to isolate the brain tissue of interest for histological investigations. Blood was obtained through cardiac puncture. The body weight result shows a significant decrease in groups B-D when compared with group A. The water maze test shows that group D had higher escape latency when compared with groups A-C. Evaluation of oxidative stress marker using MDA, GSH, SOD shows that GSH and SOD had significant decrease in groups B-D and MDA had significant increase in groups B-D when compared to control groups A. Histological result showed that, following the administration of methamphetamine, there was moderate to severe degeneraton of cells in groups B-D while, group A had active and distinct pyramidal cells. These results show that there are negative effects on the prefrontal cortex when exposed to methamphetamine....Preview Download Preview +Other Microbiology project topics and materials you might be interested in»Isolation and identification of microbes associated with spoilage bread»Bacterial analysis of urine polluted environment in federal polytechnic Nekede, Owerri»Microbiological quality of palm kernel fruits»Microbiological evaluation of home made grape juice»Therapeutic potential of Moringa Oleifera leaves»Antibacterial activity of honey on staphylococcus aureusescherichia coli and streptococcus pyogen isolated from wound»Phytochemical screening and antimicrobial assessment of Spondias Mombin (Ijikara)»Bacteriological monitoring of abattoirs in Ehime Mbano in Imo state»Proximate, selected vitamins, and minerals analysis of Moringa Oleifera»Biodeterioration of natural and artifical stone»The effect of crude avocado and pawpaw leaves extract on kidney function of guinea pigs»Isolation and characterization of fungi associated with the spoilage of corn grown in farms in Rukpokwu community»Microbial examination of raw meat obtained at main abattoir, Offa»Isolation and identification of bacterial aerosols bioaerosols dump sites in Yenagoa»Incidence of staphylococcus and salmonella species in Kunuaya retailed in Yenagoa